Dr. Ryuichiro Ishitani

Education ↑

1996-1998

Student of Faculty of Science, the University of Tokyo

1998-2003

Master and PhD student of Department of Biophysics and Biochemistry, Graduate School of Science, the University of Tokyo under Prof. S. Yokoyama

2003

Graduation from Department of Biophysics and Biochemistry, Graduate School of Science, the University of Tokyo (Doctor of Science)

Academic degree ↑

Ph.D. (Science), 2003
Title of the thesis: "Alternative tertiary structure of tRNA for recognition by a posttranscriptional modification enzyme".

Employment ↑

2003-2004

Research Associate at Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo

2004-2005

Post Doctor at Graduate School of Agricultural and Life Science, The University of Tokyo.

2005-2006

Assistant Professor at Graduate School of Agricultural and Life Science, The University of Tokyo.

2006

Consultant of A-Cube, Inc.

2006-2008

Assistant Professor at Department of Biological Information,Graduate School of Biosciences and Biotechnology,Tokyo Institute of Technology

2008-2010

Associate Professor at Department of Basic Medical Sciences,Institute of Medical Science, University of Tokyo

2011-

Associate Professor at Department of Biophysics and Biochemistry, Graduate School of Science, the University of Tokyo

Publications ↑

1. "Complete crystallographic analysis of the dynamics of CCA sequence addition."
   Tomita K, Ishitani R, Fukai S, Nureki O.,
   Nature, 7114 956-960 (2006).
   PubMed: link
2. "Structural basis of RNA-dependent recruitment of glutamine to the genetic code"
   Oshikane H, Sheppard K, Fukai S, Nakamura Y, Ishitani R, Numata T, Sherrer RL, Feng L, Schmitt E, Panvert M, Blanquet S, Mechulam Y, Soll D, Nureki O.,
   Science, 312 1950-1954 (2006).
   PubMed: link
3. "Evaluation of efficiency of reference potential spatial warping algorithm in conformational sampling of peptides"
   Ishitani R, Shimizu K, Terada T.,
   J. Chem. Phys., 124 214902 (2006).
   PubMed: link
4. "Crystallization of leucyl-tRNA synthetase complexed with tRNA^Leu from the archaeon Pyrococcus horikoshii"
   Fukunaga R, Ishitani R, Nureki O, Yokoyama S.,
   Acta Crystallogr., F61 30-32 (2005).
   PubMed: link
5. "Structural basis for template-independent RNA polymerization"
   Tomita K, Fukai S, Ishitani R, Ueda T, Takeuchi N, Vassylyev DG, Nureki O.,
   Nature, 430 700-704 (2004).
   PubMed: link
6. "Crystal structures of the CP1 domain from Thermus thermophilus isoleucyl- tRNA synthetase and its complex with L-valine"
   Fukunaga R, Fukai S, Ishitani R, Nureki O, Yokoyama S.,
   J. Biol. Chem., 279 8396-8402 (2004).
   PubMed: link
7. "The Crystal Structure of a Putative Aspartate Aminotransferase Belonging to Subgroup IV"
   Katsura Y, Shirouzu M, Yamaguchi H, Ishitani R, Nureki O, Kuramitsu S, Hayashi H, Yokoyama S.,
   Proteins, 55 487-492 (2003).
   PubMed: link
8. "Divergent evolutions of trinucleotide polymerization revealed by an archaeal CCA-adding enzyme structure."
   Okabe M, Tomita K, Ishitani R, Ishii R, Takeuchi N, Arisaka F, Nureki O, Yokoyama S.,
   EMBO J., 22 5918-5927 (2003).
   PubMed: link
9. "Activation of the pyrrolysine suppressor tRNA requires formation of a ternary complex with class I and class II lysyl-tRNA synthetases."
   Polycarpo C, Ambrogelly A, Ruan B, Tumbula-Hansen D, Ataide SF, Ishitani R, Yokoyama S, Nureki O, Ibba M, Soll D.,
   Mol. Cell, 12 287-294 (2003).
   PubMed: link
10. "Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic-code expansion"
    Kobayashi T, Nureki O, Ishitani R, Yaremchuk A, Tukalo M, Cusack S, Sakamoto K, Yokoyama S.,
    Nat. Struct. Biol., 10 425-432 (2003).
    PubMed: link
11. "Alternative tertiary structure of tRNA for recognition by a posttranscriptional modification enzyme"
    Ishitani R, Nureki O, Nameki N, Okada N, Nishimura S, Yokoyama S.,
    Cell, 113 383-394 (2003).
    PubMed: link
12. "Crystal structure of human epidermal growth factor in complex with the extracellular domains of the receptor"
    H. Ogiso, R. Ishitani, O. Nureki, S. Fukai, M. Yamanaka, J.-H. Kim, K. Saito, M., Shirouzu, and S. Yokoyama,
    Cell, 110 775-787 (2002).
    PubMed: link
13. "Crystal structure of the homologous-pairing domain from the human Rad52 recombinase in the undecameric form."
    Kagawa W, Kurumizaka H, Ishitani R, Fukai S, Nureki O, Shibata T, Yokoyama S.,
    Mol. Cell, 10 359-371 (2002).
    PubMed: link
14. "An enzyme with a deep trefoil knot for the active-site architecture"
    Nureki O, Shirouzu M, Hashimoto K, Ishitani R, Terada T, Tamakoshi M, Oshima T, Chijimatsu M, Takio K, Vassylyev DG, Shibata T, Inoue Y, Kuramitsu S, Yokoyama S.,
    Acta Crystallogr., D58 1129-1137 (2002).
    PubMed: link
15. "Functional convergence of two lysyl-tRNA synthetases with unrelated topologies"
    Terada T, Nureki O, Ishitani R, Ambrogelly A, Ibba M, Soll D, Yokoyama S.,
    Nat. Struct. Biol., 9 257-262 (2002).
    PubMed: link
16. "Crystal structure of archaeosine tRNA-guanine transglycosylase"
    Ishitani R, Nureki O, Fukai S, Kijimoto T, Nameki N, Watanabe M, Kondo H, Sekine M, Okada N, Nishimura S, Yokoyama S.,
    J. Mol. Biol., 318 665-677 (2002).
    PubMed: link
17. "Selenomethionine incorporation into a protein by cell-free synthesis"
    Kigawa T, Yamaguchi-Nunokawa E, Kodama K, Matsuda T, Yabuki T, Matsuda N, Ishitani R, Nureki O, Yokoyama S.,
    J. Struc. Func. Gen., 2 87-104 (2001).
    PubMed: link
18. "Crystallization and preliminary X-ray analysis of archaeosine tRNA-guanine transglycosylase from Pyrococcus horikoshii"
    Ishitani R, Nureki O, Kijimoto T, Watanabe M, Kondo H, Nameki N, Okada N, Nishimura S, Yokoyama S.,
    Acta Crystallogr., D57 1659-1662 (2001).
    PubMed: link